Asian Journal of Complementary and Alternative Medicine

Evaluation of Antihyperglycemic and Hypolipidemic Activities of Clerodendrumn infortunatum Linn. Leaf Extracts

Baid S.S.

Baid S.S.

Abstract

Ethnopharmacological relevance: Clerodendrum infortunatum Linn. (Verbenaceae), commonly known as Bhant in Hindi, has been traditionally used in the Siddha system of medicine as a chief ingredient of many polyherbal formulations for the treatment of diarrhoea, rheumatic complaints, diabetes, fever and skin ailments. However, no scientific evidence is available to validate the folklore claim. The present study has been designed to evaluate its leaf extract for the antidiabetic and hypolipidemic activity. Aim: To evaluate leaves of Clerodendrum infortunatum for the antihyperglycemic and hypolipidemic activity. Materials and methods: Different leaf extracts of Clerodendrum infortunatum were evaluated for the polyphenol, flavonoid, steroid contents and in-vitro antihyperglycemic activity (alpha-glucosidase inhibition assay). On the basis of Quantitative estimation and in vitro alpha-glucosidase inhibition assay, chloroform extract (CECI) was screened for the antihyperglycemic activity by streptozotocin induced diabetic rats and biochemical parameters viz. total cholesterol, TG, HDL cholesterol and LDL cholesterol were also evaluated for hypolipidemic activity. Results: Chloroform extract (CECI) at the doses of 200 and 400 mg/kg significantly (P<0.05) and dose-dependently reduced and normalized blood glucose levels as compared to that of the STZ control group. Serum biochemical parameters were significantly (P<0.05) restored towards normal levels in CECI-treated rats as compared to the STZ control. In order to assess the role of secondary metabolites in the relevant activity, polyphenolic, flavonoid and steroid contents were determined. The highest polyphenolic and flavonoid content were found in hydroalcoholic extract 58.78 μg/mg (GAE mg/g of extract), 73.84 μg/mg (rutin equivalent/g extract) respectively. The chloroform extract has shown highest steroid content 17.63 μg/mg and α-glucosidase inhibition activity. Conclusion: The present study demonstrated that the leaves of C. infortunatum had remarkable preclinical antihyperglycemic activity in STZ-induced diabetic rats. Our results contribute towards validation of the traditional use of C. infortunatum in the treatment of diabetes. Keywords: Antihyperglycemic, Clerodendrum infortunatum, Streptozotocin, Metformin

Keywords:

Antihyperglycemic, Clerodendrum infortunatum, Streptozotocin, Metformin