Antipyretic Effect of Sophora Flavescens (Kushen) Decoction on Dry Yeast-Induced Fever in Rats
Eerdunchaolu1,2, Minghai Fu1,2 and Sungbo Cho2*
1NMPA Key Laboratory of Quality Control of Traditional Chinese Medicine (Mongolian Medicine), Inner Mongolia University for Nationalities, Tongliao 028000, China
2School of Mongolian Medicine, Inner Mongolia University for Nationalities, Tongliao 028000, China
Objective: The current study investigated the effect of Sophora flavescens, known as Kushen decoction (KSD) on body temperature and metabolism against fever induction in Wistar rats.
Methods: Firstly UPLC-Q-TOF/MSE analysis was carried out for identifying the KSD composition. Fever model was induced by dry yeast and, orally administered at dose of KSD (500 mg/kg) to the rats. The alteration of the rectal temperature (TR) was estimated to evaluate the positive effect of KSD. TR changes were recorded over time. In addition, the antipyretic effect was confirmed from the hypothalamus of rats through real-time PCR.
Results: The model and treatment groups showed significantly increased TR and the mRNA expression of IL-1 and IL-6 after 5 hours, even though the expression level of arginine vasopressin (AVP) and TNF-alpha were not altered. The TR tests indicated the administration of KSD to fevered rats attenuated yeast-induced fever and, significantly reduced PGE2 expression levels in hypothalamus. The mRNA expression results reveal that dry yeast-induced fever could be mediated by PGE2 production.
Conclusions: KSD could play the antipyretic effect by reducing PGE2 expression in the hypothalamus. Subsequently, KSD inhibited the development of yeast-induced fever.
Sophora flavescens decoction; Antipyretic effect; PGE2; Chemical composition