Biography
Dr. Abu Musa Md Talimur Reza
Institute of Biochemistry and Biophysics Polish Academy of Sciences
Poland
Institute of Biochemistry and Biophysics Polish Academy of Sciences, Poland
Adiunkt (Assistant Professor): March 1, 2018 to Current
Department of Genetics.
Adiunkt (Assistant Professor): March 1, 2018 to Current
Department of Genetics.
Research Interests
Cell & Molecular Biology
1. Primary Cells - isolation and culture of adipose, muscle and cartilage cells
2. Cell Lines - Cancer cells, Human, Mouse and Bovine cells, iPS cells (feeder free)
3. Assays - Commonly used cell biology assays including proliferation, live-dead,
migration, colony formation etc.
4. CRISPR/Cas9 sgRNA design, Oligo insertion, Insert checking, Amplification and
cloning of vector
5. Genotyping (Direct sequencing part was done from company)
6. Plasmid and Genomic DNA, mRNA and miRNA, Protein sample preparation
7. Exosome isolation and characterization
8. Lipofectamine mediated transfection of plasmid DNA and miRNA
9. qRT-PCR, RNA-hybridization, Western Blotting, Immunostaining, FACS
(CytoFLEX and BD FACScalibur), MACS, Fluorescence Microscopy
Sequencing and Bioinformatics
1. Library preparation: Ribosome profiling (Ribo-seq) and RNA-seq
2. Some primary analysis using ‘R-statistics’
3. Microarray and RNA-seq secondary data analysis using different analytical program
such as DAVID, PANTHER, CytoScape, IPA
4. Use of publicly available database such as TCGA cBioPortal database system
Mouse handling
1. Sacrificing and tissue collection such as thymus, spleen, lymph node, liver, kidney,
lung, heart, brain, intestine, stomach, adipose, muscle etc.
2. Epididymal sperm collection and cryopreservation
3. Xenotransplantation (under skin injection of cancer cells using matrigel)
4. Intraperitoneal injection
5. Tail vein injection (not very efficient, need practice)
Other Techniques
1. Nanoparticles preparation
1. Primary Cells - isolation and culture of adipose, muscle and cartilage cells
2. Cell Lines - Cancer cells, Human, Mouse and Bovine cells, iPS cells (feeder free)
3. Assays - Commonly used cell biology assays including proliferation, live-dead,
migration, colony formation etc.
4. CRISPR/Cas9 sgRNA design, Oligo insertion, Insert checking, Amplification and
cloning of vector
5. Genotyping (Direct sequencing part was done from company)
6. Plasmid and Genomic DNA, mRNA and miRNA, Protein sample preparation
7. Exosome isolation and characterization
8. Lipofectamine mediated transfection of plasmid DNA and miRNA
9. qRT-PCR, RNA-hybridization, Western Blotting, Immunostaining, FACS
(CytoFLEX and BD FACScalibur), MACS, Fluorescence Microscopy
Sequencing and Bioinformatics
1. Library preparation: Ribosome profiling (Ribo-seq) and RNA-seq
2. Some primary analysis using ‘R-statistics’
3. Microarray and RNA-seq secondary data analysis using different analytical program
such as DAVID, PANTHER, CytoScape, IPA
4. Use of publicly available database such as TCGA cBioPortal database system
Mouse handling
1. Sacrificing and tissue collection such as thymus, spleen, lymph node, liver, kidney,
lung, heart, brain, intestine, stomach, adipose, muscle etc.
2. Epididymal sperm collection and cryopreservation
3. Xenotransplantation (under skin injection of cancer cells using matrigel)
4. Intraperitoneal injection
5. Tail vein injection (not very efficient, need practice)
Other Techniques
1. Nanoparticles preparation