Medicinal foods YT and RH suppress cigarette smoke-induced inflammation and oxidative stress by inhibiting NF-κB/ERK signaling pathways
Yuanyuan Li1,2, Lan Wang1,2, Qing Gao3, Jieping Luo1, Yu qing Chen1, Jingyi Xu1, Guofa Zou1, Yanran Zhan1, Meiyin Wu4, Hanyong Chen3, Zhen Luo3, Wenju Lu1,2*
1 Guangzhou Medical University, Guangzhou, Guangdong, PR China;
2 State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, PR China;
3 Infinitus (China) Co., Ltd, Guangzhou, Guangdong, PR China;
4 Guangzhou Hongyun Medical Scientific and Technological Co., Ltd, Guangzhou, Guangdong, PR China
Background: Cigarette smoke is a risk factor for COPD. Given the lack of a COPD curative treatment, dietary management of COPD patients has become important. This study investigated whether the medicinal foods (YT and RH) could suppress cigarette smoke exposure-induced inflammation and oxidative stress.
Methods: Chronic pulmonary inflammation in male C57 mice were induced by 4 weeks cigarette smoke (CS) exposure. The medicinal foods YT and RH were orally administered to mice 1 week prior to CS exposure. The protective effects were assessed by measuring the pulmonary function and histopathological evaluations. Inflammatory cell numbers and cytokines levels in BALF and blood serum were analyzed by enzyme-linked immunosorbent assay (ELISA). MDA and SOD levels of lung were analyzed. Furthermore, The levels of phosphorylated ERK and NF-κB of mice lungs and RAW264.7 cells were also detected.
Results: YT and RH combination significantly improved pulmonary function, and suppressed the inflammation including cell number and cytokines in BALF relative to the CS group, histological examination revealed protective effect of YT and RH combination in the lungs of mice exposed to CS. Moreover, the MDA level of YT and RH combination group mice lung was lower and the SOD activity was higher, in vitro YT and RH combination attenuated ROS expression in mouse macrophage RAW264.7 cells stimulated with CSE. YT+RH combination significantly reduced the expression of pNF-κB and pERK in the lung tissues and macrophage stimulated with CSE.
Conclusion: YT and RH combination attenuates cigarette smoke induced inflammation and oxidative stress through inhibition of NF-κB/ERK signaling pathway activation.
YT; RH; cigarette smoke; inflammation; oxidative stress; NF-κB/ERK