Asian Journal of Complementary and Alternative Medicine

Asian Journal of Complementary and Alternative Medicine

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Improve the intestinal bacterial flora environment on renal function recovery in chronic kidney disease by efficacy of administration of plant enzymes

Yeun-Hwa Gu*1, Takenori Yamashita2, Tatsuhiko Matsuo3, Noriyuki Washino4, Jin-Ho Song5, Ki-Mun Kang6, Tota Inoue7

1Department of Radiological Science, Faculty of Health Science, Junshin Gakuen University, Fukuoka, Japan.

2Department of Radiological Science, Faculty of Health Science, Suzuka University of Medical Science, Mie, Japan.

3Managing Director, Validux Co., Ltd. Nagoya, Japan

4General Manager, Research Institute for Ishokudogen, Mie Pref. Japan

5Department of Radiation Oncology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

6Department of Radiation Oncology, Gyeongsang National University School of Medicine and Gyeongsang National University Changwon Hospital, Changwon, Republic of Korea.

7Mie breathing swallowing rehabilitation clinic, Mie, Japan


In renal failure, it is believed that decreased intestinal barrier function is caused by deterioration of the intestinal microflora environment and by intestinal mucosal disorder. This deterioration further promotes chronic kidney disease and unavoidably causes cardiovascular disorders and kidney death. Accumulation of uremic toxins also occurs. In this study, we investigated the effect of improving the intestinal microflora environment by administration of plant enzymes on chronic kidney damage, one of the sequelae of type 2 diabetes.

We examined the effect of administering plant enzymes in renal disorder model mice. The concentration of plant enzymes was 500 mg/kg/day, and the same amount of distilled water was administered to the control group. We administered these enzymes to mice by oral administration for over 2 weeks.

The control group received (Please provide the same type of information as above). Intestinal function was assessed by examining intestinal immunity and gastrointestinal action. Kidney function was assessed by histopathological analysis of glomerular tissue of the kidney and by measuring mouse blood and urinary protein levels.

Gut-associated lymphoid tissue function, including intestinal lymphocyte count and secretory immunoglobulin A level, was decreased, which led to restoration of kidney function. Histological findings of the glomerular tissue of the kidney showed restoration of kidney function. The mouse blood and urinary protein test results showed decreased urinary protein levels at 5–7 weeks relative to the levels in the control group.

Administration of plant enzymes improved the intestinal microflora environment, which led to improved intestinal function and reduction in the molecular weights of proteins at the nutrient absorption stage, which reduced the burden on the kidneys. Consequently, renal function recovered. The mice were treated to induce renal injury and examined how the gut microbiota changed to ensure that certain gut bacteria changed. In this study, it was found that some protective factors for the kidney are produced from intestinal bacteria.


Sequelae of type 2 diabetes, Kidney function, Urinary proteins, plant enzyme, Blood urea nitrogen, Creatinine

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