Asian Journal of Complementary and Alternative Medicine

Evaluation of in vivo wound healing and anti-inflammatory activity of 80% methanolic extract of the leaves of B. antidysentrica J. F. Mill (Simaroubaceae) in mice

Zenaw Tessema¹, Eyasu Makonnen², Asfaw Debella³, Desalegn Yibeltal⁴ and Yalew Molla⁵

¹Department of Pharmacy, College of Health Sciences, Debre Markos University, P. O. Box, 269, Debre Markos, Ethiopia.

²Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Addis Ababa University, P. O. Box, 1176, Addis Ababa, Ethiopia,

³Traditional and Modern Medicine Research Directorate, Ethiopian Public Health Institute.P.O.Box, 1242, Addis Ababa, Ethiopia.

⁴Department of Pharmacy, College of Health Sciences, Debre Markos University, P. O. Box, 269, Debre Markos, Ethiopia.

Abstract

Objective: The purpose of this study was to evaluate the wound healing and anti-inflammatory activities of the crude extract of the leaves of B. antidysentrica J. F. Mill.

Results: Both 2% and 4% crude extract revealed significant wound healing activity compared with negative control as evidenced by an increase in the percent of wound contraction (p < 0.01) and a decrease in epithelization period (p<0.05). In addition, both 2% and 4% extract ointments resulted in a significant increase in tensile strength (p < 0.01) compared with negative control. Hundred, two hundred and four hundred mg/kg extracts of the leaves exhibited a higher anti-inflammatory effect on the 3^rd (P<0.05) and 4^th hour (P<0.001) as compared to the negative control.

Keywords:

B. antidysentrica J. F. Mill, wound healing, anti-inflammatory, excision, incision